Bioinformatics research inevitably requires a powerful computing facility. This is because Bioinformatics analysis involves a large sequence dataset. Pawsey Supercomputer Centre, Perth, offers a great solution to it. The centre provides with a custom-tailored computing infrastructure to all Australian researchers. It also has a strong support team comprised of professional Bioinformaticians to help researchers along with their computing journey.
Pawsey Supercomputer Centre has two types of computing facilities- high performance computing (HPC) and cloud based virtual machine called ‘Nimbus Instance’.
Here, I outline the steps to get connected to Pawsey's ‘Nimbus Instance’.
Step 1: Make an application to have access to ‘Nimbus Instance’ (https://apply.pawsey.org.au/)
Step 2: Log in into https://nimbus.pawsey.org.au and build an instance
As a part of this step, you will generate ‘a key pair’ that will be required for ssh connection via any ‘secure shell (ssh)’ client (software) (https://pawsey.atlassian.net/wiki/spaces/US/pages/51915082/Create+a+Nimbus+Instance)
Step 3: Attach a storage volume to your Instance
Instructions are here:
Step 4: Access your Instance through a ssh client
To access the Pawsey’s Nimbus Instance, see the instructions in the following link:
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In your Instance, you have two IP addresses: one Instance-specific and one external. You need the external IP address in this step
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You need to add an inbound and outbound rule for Port 22 to your local computer’s firewall
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Pay attention to the remote host name. It is not just the IP address but ubuntu@IP_address
Here is an example of connecting to Pawsey’s Ubuntu Instance using MobaXterm:
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See the MobaXterm screenshot below (Fig. 1)
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Enter remote host as
ssh ubuntu@Public_external_IP_for_your_instance
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Tick the ‘Specify username’ box. It is the username that you use to log in into your Nimbus Instance website (dashboard)
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Tick the ‘Use private key’ box. Browse to your key. It is a file with ‘.pem’ extension. Select it and press OK.
Now, ssh connection established and you can use your Instance
Figure 1. ssh connection to Nimbus Instance via MobaXterm
Step 5: Transfer your data to your Instance that you want to analyse
Data can be transferred back and forth between your ‘local computer’ and ‘Instance’ using one of the following options:
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command line in your shell
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MobaXterm’s drag and drop function, or
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a software, for example, WinSCP, FileZila etc.
You need to download it and log in into it with the following details:
Host: same as Step 4, Username and Password: same as your Pawsey account, Port: 22, Passphrase: the one that you have generated with Putty at Step 4
The End